Issue:ISSN 2095-1353
CN 11-6020/Q
Director:Chinese Academy of Sciences
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2009年46 No.5
Establishment of cell line of Musca domestica embryo
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Key Words：Musca domestica, embryo, cell line, biological characteristics
Abstract：Insect cell lines have become useful tools in many research fields. Em
bryos of Musca domestica L. were used as experimental materials. The cells w
ere cultured in M3 medium containing 20% fetal bovine serum. After about 35day primar
y culture, the cells could be passaged one time every 7 days, and were stably pas
saged for 60generation period. The time of the cell population doublings was 4
4 h. The chromosomes are 2n=12. The cells from this cell line could be stored at
liquid nitrogen or 4℃ refrigerator, and resuscitated at room temperature. It was
named MDEC-07114.