Phylogenetic analysis of COⅠ and COⅡ gene sequences of different splash types of Harmonia axyridis.
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Key Words：Harmonia axyridis, mtDNA, COⅠ, COⅡ
Abstract： Phylogenetic analysis of 13 species splash types of Harmonia axyridis Pallas was conducted based on mitochondrial COⅠ and COⅡ DNA sequences. The fre q uency of nucleotide usage was calculated and the molecular phylogenetic tree was constructed in order to explore the regular pattern of the variation of H. axyridis splash types. The results revealed that there were 31 variable site s and 29 parsimonyinformation sites in COⅠ and COⅡ gene sequence fragments, an d m ean A+T content was about 71.2%, most of which were transformed through transve r sion. By using NeighborJoining (NJ) , Maximum Parsimony (MP) and Minimum Evolu t ion (ME) methods, we constructed the molecular phylogeny. The genetic distance a mong different splash types of H.axyridis was 0.0021 to 0.0328 The aver age gene tic distance was 0.0193 The molecular phylogenetic tree suggested that the me mb ers with the same elytron color were grouped together with high bootstrap confid ence values, showing low diversity among the populations. H. axyridis ab. luna t a Hem and H.axyridis var. spectabilis Fald were most closely related. H.ax yridis var. axyridis Pallas was a primordial population of coccinellidae, which wa s differentiated earlier than the other populations.