Cloning and sequence analysis of alkaline phosphatase gene in the diamondback moth, Plutella xylostella.
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Key Words：Plutella xylostella, alkaline phosphatase, cloning, sequence analysis
Abstract： Alkaline phosphatase genes were cloned from the resistant and susceptible strains of Plutella xylostella(L.) through RT-PCR technique using degenerate primers and sequenced. The cDNA length was 403 bp and the sequence was confirmed to be the alkaline phosphatase gene by blast alignment(BLAST). The nucleotide similarity of the resistant and susceptible strains was 99.5% and the amino acids identity was 99%. Compared with the susceptible strain, 18 nucleotides were changed in the resistant strain, resulting in one amino acid changed (V to T). These results are helpful for further study of the full sequence and function of alkaline phosphatase gene in P. xylostella.