Phylogenetic analysis of Actias selene based on 18S ribosomal RNA and mitochondrial 16S rRNA genes
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Key Words：Actias selene, 18S ribosomal RNA, mitochondrial 16S rRNA, phylogenetic relationship
Abstract： To investigate the phylogenetic relationship between Actias selene Hübner and other Lepidopteran insects, a 391 bp fragment of 18S ribosomal RNA and a 428 bp fragment of mitochondrial 16S rRNA were used to construct phylogenetic trees with neighbor\|joining (NJ), maximum parsimony (MP) and arithmetic averages (UPGMA) methods. The results showed the nucleotide composition of mitochondrial 16S rRNA gene of A. selene had AT bias, similar in other Saturnidae insects. The genetic distances between A. selene and other species ranged from 0.016 to 0.140 based on mitochondrial 16S rRNA gene sequences, in which the minimum distance was that to Antheraea roylii and the maximum was that to Bombyx mandarina. As far as 18S rRNA gene sequences were concerned, the minimum distance (0.010) and the maximum distance (0.017) were showed between A. selene with A. pernyi, and Samia ricini, respectively.