Cloning and analysis of small heat-shock gene Hsp23.7 from Bombyx mori

Key Words： heat shock protein family，tissue expression pattern， Real-time PCR Bombyx mori，Bm-Hsp23.7 gene，small

Abstract：        Ｈsp23.7 is one of the small heat-shock family of proteins. The Hsp23.7 gene of Bombyx mori L. (Bm-Hsp23.7) was cloned and expressed in E. coli. The candidate ORF of Bm-Hsp23.7 contains 210 amino acids. Its molecular weight (mw) is 23.7 ku and isoelectric point (pI) is 5.17. Real-time PCR data was used to reveal the profile of the spatio-temporal expression pattern of Bm-Hsp23.7. Absolute quantization demonstrated that Bm-Hsp23.7 was expressed at different levels in all tissues of 5th instar larva of B. mori. The highest level of expression was in the ovary (3.64×107 copy numbers/μg), followed by the fat bodies, then the wing discs and malpighian tubules. The lowest level of expression was in hemocytes (7.11×103 copy numbers/μg).