The genetic divergence of nine populations of Oedaleus asiaticus in Mongolia based on RAPD
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Key Words:Oedaleus asiaticus,RAPD,genetic divergence
Abstract: The genetic divergence of 9 natural populations of Oedaleus asiaticus (B.-bienko) was studied using the RAPD method. Genomic DNA of 90 individuals was amplified with eight random primers. A total of 78 clear and reproducible bands were generated, 62 of which were polymorphic, and Nei's index and a genetic distance matrix calculated. The gene differentiation coefficient between populations estimated by Nei' s index was 0.2343, indicating that 23.43% of the variation occurred between populations. The gene differentiation coefficient within populations was 0.7657, indicating that 76.57% of all variation was within populations. The gene differentiation coefficient within populations was evidently higher than that between populations. A dendrogram based on Nei's genetic distance of RAPD markers was constructed using the NJ method. The results indicate that genetic divergence in populations of O. asiaticus corresponds to both geographic and genetic distance.