Isolation and identification of bacteria producing enzymes from gut and skin of black soldier fly（Hermetia illulens）larvae.

Key Words： black soldier fly, companion microbe, protein catabolism, organic phosphate catabolism, Bacillus subtilis

Abstract：       Ten bacterial strains were isolated from the gut, or skin, of the black soldier fly (Hermetia illucens L.) larvae, collected from the field or when feeding indoors. The strains from the gut were named S14, S15, S16, S19 and S20, and those from skin were named T2, T4, T6, T7 and T8. All strains had both protein and organic phosphate metabolic capability. The 16S rDNA sequences of these strains were cloned; BLAST analytical results identified all strains as Bacillus species. The gyrB gene, which encodes DNA gyrase B protein, was used to identify the strains to the species level; all strains were identified as B. subtilis. A phylogenetic tree based on the gyrB gene sequences of the ten strains, B. subtilis subsp. subtilis str.168 and strain BCRC10255, indicates that the ten B. strains have phylogenetic relationships and that each is unique.