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Your Position :Home->Past Journals Catalog->2011年48 No.2

Detection of midgut apoptosis in Nilaparvata lugens larvae
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Key Words:wordsNilaparvata lugens, midgut, apoptosis
Abstract:        Midgut apoptosis was investigated in the brown planthopper (Nilaparvata lugens Stal, Hemiptera: Delphacidae) larvae. A terminal deoxynucleotidyltransferasemediated dUTPbiotin nick end labeling (TUNEL) assay revealed that two to five percent of the 1st-5th larval instar midgut cells showed typical fluorescent signals representing genomic DNA cleavage. 4′, 6-diamidino-2-phenylindole (DAPI) staining revealed apoptotic nuclei presenting characteristic features in apoptosis stage Ⅰ, Ⅱa and Ⅱb; including chromatin condensation, margination and nuclear fragmentation. Transmission electron microscopy revealed that early stage apoptotic midgut cells had apoptotic nuclear morphology, such as chromatin condensation and margination, while late stage cells had broken nuclei, apoptotic bodies and vacuolization of the cytoplasm. These findings demonstrate that a small percentage of the midgut cells of N. lugens larvae
undergo apoptosis during normal development. It may be possible to regulate N. lugens midgut apoptosis artificially thereby making this process a novel method of control for this species.
 
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