Method improvement for extraction genomic DNA from thrips
Author of the article:
Author's Workplace:
Key Words:thrips, DNA extraction, PCR
Abstract: To isolate total DNA from insect samples is a crucial prerequisite for successful PCR reactions. However, regular methods for isolating insect DNA can’t resolve all the morphological characters of insects. Because these methods destroy insects’ morphological characteristics they are obviously not suitable for scarce and small insects such as thrips. In this article, two methods of saltingout and the STE method of extracting genome DNA from individual thrips are compared to KAc. The results suggest that,the quality of DNA samples extracted by the saltingout and STE methods was obviously better and more suitable for PCR than those obtained by the KAc method. Genome DNA was extracted from individual thrips by piercing one side of the specimen’s abdomen with a minute, sterilized pin, thereby avoiding destroying their morphological features. Experiments demonstrated that the extracted DNA was suitable for PCR amplification using the sequencing primers Mitochondrial Cytochrome Oxidase I and the ITS2 region of ribosomal DNA.