The RNA interference efficiency of glutathione Stransferases from Locusta migratoria manilensis
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Key Words：glutathione Stransferases (GSTs), Locusta migratoria manilensis, RNAi, time effect
Abstract：T he migratory locust, Locusta migratoria manilensis (Meyen) (Orthoptera: Acridoidea), is one of the most important pests in our country. Resistance of this locust to some insecticides has been detected and the resistance mechanism hypothesized to involve glutathione Stransferases (GST). In this study, doublestranded RNA of four different classes of GST was synthesized using specific gene primers and injected into the 2nd instar nymphs of L. m. manilensis. The effects of silencing time on target GSTs mRNA expression was assayed by real time RT-PCR. The results show that the expression of LmGSTd1 and LmGSTs5 were reduced significantly 12 h after injection, whereas LmGSTt1 and LmGSTu1 were reduced significantly 24 h after injection. These results provide a foundation for further study of GST function and mechanisms in locusts.