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Your Position :Home->Past Journals Catalog->2011年48 No.4

Comparison among three staining methods to hemocytes of Oxya chinensis
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Key Words:Oxya chinensis, hemocytes, staining method
Abstract:
       The staining efficiency of the Giemsa, Wright and WrightGiemsa staining methods were compared with respect to hemocytes of Oxya chinensis(Thunberg). The Giemsa method effectively stained the nucleus; cytoplasm boundaries were clear, but it was relatively time consuming and did not stain cytoplasmic granules very well. The Wright method effectively stained both nucleus and cytoplasmic granules in a relatively short time, but cytoplasm boundaries were not clear. The WrightGiemsa method had the advantages of both the Giemsa and Wright methods, including shorter staining time, good staining of nucleus and cytoplasmic granules and clear cytoplasm boundaries. This method should be used more widely in studying insect hemocytes.
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