Latest Cover

Online Office

Contact Us

Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Tel:+86-10-64807137
Fax:+86-10-64807137
Email:entom@ioz.ac.cn
Your Position :Home->Past Journals Catalog->2012年49 No.5

The detection method of intracellular Ca2 + ion concentrationof brain neurons of honeybee Apis mellifera ligustica
Author of the article:
Author's Workplace:
Key Words:honeybee,brain cells,Fura-2 AM,free calcium concentration,determination
Abstract:
  • A prototype in vitro method to measure intracellular free calcium concentration ([Ca2 +]i ) in honeybee brain
    cells was developed and tested. Fura-2 AM was used as a florescence indicator to measure intracellular calcium ion
    concentration ratio values and incubation time ratio was also recorded and analyzed using META FLOUR software. The
    results revealed that cell ratio values decreased among groups with increased concentration of Fura-2 AM during cell
    incubation,and there were highly significant differences between groups ( P < 0. 01 ) . Similarly,it was clear that
    incubation time can potentially affect ratio values; for example only 10 mins incubation resulted in ratio values significantly
    lower than the other treatments.
CopyRight©2024 Chinese Journal of Aplied Entomology