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2012年49 No.5
The detection method of intracellular Ca2 + ion concentrationof brain neurons of honeybee Apis mellifera ligustica
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Key Words:honeybee,brain cells,Fura-2 AM,free calcium concentration,determination
Abstract:
- A prototype in vitro method to measure intracellular free calcium concentration ([Ca2 +]i ) in honeybee brain
cells was developed and tested. Fura-2 AM was used as a florescence indicator to measure intracellular calcium ion
concentration ratio values and incubation time ratio was also recorded and analyzed using META FLOUR software. The
results revealed that cell ratio values decreased among groups with increased concentration of Fura-2 AM during cell
incubation,and there were highly significant differences between groups ( P < 0. 01 ) . Similarly,it was clear that
incubation time can potentially affect ratio values; for example only 10 mins incubation resulted in ratio values significantly
lower than the other treatments.