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Your Position :Home->Past Journals Catalog->2012年49 No.5

Using phage display to map the binding epitope of the Bacillus thuringiensis Cry2Ab toxin
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Key Words:phage display,epitope mapping,Cry2Ab
Abstract:The insecticidal Cry toxins produced by Bacillus thuringiensisare highly specific to different insects Various proteinssuch as cadherinaminopeptidase-N (APN ) and alkaline phosphatase (ALP ) are characterized as potential Cry-receptors Howeverlittle is known about the mode of Cry2Ab actionsuch as its receptors and binding sites We used phage display to characterize the binding epitope of the Cry2Ab toxinin vitro A two peptide sequence was identified after four-rounds of screening ELISA analysis showed that activated Cry2Ab toxin could bind these two peptides with highaffinity The results indicate that employing this method can efficiently screen out target peptides with high affinity andspecificityThe method also provides a valuable platform to discover the mode of other Bt toxins
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