Issue:ISSN 2095-1353
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2012年49 No.6
Cloning and sequencing analysis of the codling mothCydia pomonella COⅠ gene
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Key Words：codling moth，Cydia pomonella，COⅠ gene，clone，sequence analysis
Abstract：
The mitochondrial COⅠ gene fragment of codling moth Cydia pomonella ( L． ) specimens from the Alar area ofXinjiang was amplified via PCR and the PCR products were purifiedclonedandsequenced． 709 bp nucleotide sequencesere obtained with a very high A + T content (68. 7% ) and relatively low G + C ontent (31. 3% ) ． Comparing the COⅠsequence of the codling moth with that of several other moth species，the homologies of nucleotides ranged from 85. 4% to88. 1% and the genetic distances ranged from 0. 130 to 0. 162. Reconstructing the molecular phylogenetic tree of theTortricidae with the Neighbor-Joining ( NJ ) method produced a result in accord with the raditional morphologicalclassification． The results of this study provide an importantbasisfortherapididentification of C． pomonella with DNAbarcode technology．