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2012年49 No.6
Amplified ribosomal DNA restriction analysis and identification ofintestinal aerobic bacteria from 4th instar larva of
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Key Words：Dendrolimu kikuchii，intestinal aerobic bacteria，diversity，ARDRA
Abstract：11 strains of aerobic bacteria were isolated from the intestine of Dendrolimu kikuchii Matsumura using the
plating method． Bacterial genomic DNA was used as templates． A new method of analyzing the genetic diversity ofintestinal aerobic bacteria was established using the PCR technique ( primer27f，1492r) and ARDRA patterns，whichused enzyme digestion (HaeⅢ and HindⅢ，HinfⅠ and TaqⅠ) of cloned 16S rDNA gene sequences． The results showthat 11 strains could be divided into 6 groups at a similarity level of 84% ． The results prove that Dendrolimu kikuchii hasa genetically diverse，aerobic，intestinal bacterial fauna． Analysis of full-length 16S rDNA sequences from representativestrains of the identified bacterial groups showed that these strains belong to Klebsiella sp． ( 4N05，4N08 and 4N11 )，Lysinibacillus sp． (4N02)，Gamma proteobacterium (4N03)，Bacillus subtilis strain (4N09)，Brevibacillus sp． (4N04，4N06，4N07，4N01 and 4N10 ) ． This study provides a theoretical basis for the microbiological control ofDendrolimu
kikuchii and enriches the microbial resources library．，identification.