Rapid identification of aphids on flowering plants of Rosaceae in Beijing based on DNA barcoding
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Key Words：aphid, DNA barcoding, COⅠ, Rosaceae flower plant
Abstract： Flowering ornamental plants of the Rosaceae family are a major feature of Beijing’s gardens but are also host plants for a variety of aphids. It’s often difficult to identify aphids rapidly and accurately by traditional methods due to their tiny bodies，degradation and specialization of morphological characteristics, and the occurrence of multiple morphs within single species. We evaluated DNA barcoding based on COⅠ sequences as a means of rapidly identifying the aphids found on Rosaceae flowering plants in Beijing. In this study, 65 COⅠ sequences were sequenced, including 12 species from 9 genera. The average intraspecific divergence was 0.70% whereas interspecific divergence was 9.45%\|12.59%. NJ/MP trees based on COⅠ sequences indicated that most samples of the same species clustered together with strong support (bootstrap value > 90%). From the combination of sequence divergences and phylogenetic trees, DNA barcoding reliably identified 83% of the aphid species.