This paper reviews strategy and methods for sequencing mitochondrial genomes. Two traditional sequencing methods are introduced; the clone librarybased sequencing method from physically purified mtDNA, and the PCR ampliconbased sequencing method, which includes primerwalking sequencing from long PCR amplicons and primerwalking sequencing from total DNA. For the application of nextgeneration sequencing strategies, there are total DNAbased methods, which include the parallel tagged sequencing (PTS) method based on preamplifying mtDNA and total DNA, and the transcriptomic sequencing method based on total RNA. From a practical perspective, which method is chosen depends on the scale of the research, size and preservative status of the sample, and financial support. The primer walking sequencing method based on longPCR amplicons is particularly suitable for small scale research on insect mitochondrial genomes. For large scale mitochondrial genome research, NGS technology is the best choice for saving time and effort.