Issue:ISSN 2095-1353
CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
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2013年50 No.1
DNA barcoding in rapid identification of aphids on Pinus armandii
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Key Words：Pinus armandii, aphids, DNA barcoding, fuzzy type, rapid identification
Abstract：
- DNA barcoding is a technique for identifying species using a short sequence of DNA. DNA barcoding combined with morphometry can be used to rapidly identify a large number of specimens when there is insufficient information for full species identification. Aphids are one of the most common pests of Pinus armandii Franch, an important endemic Chinese timber species. Rapid and accurate identification of these pests can contribute to effective prevention and control. This paper evaluates the usefulness of DNA barcoding with respect to aphids on P. armandii, Neighbour\|Joining (NJ) tree was constructed based on mitochondrial COⅠ sequences. Aphids from different locations on P. armandii formed 13 distinct clusters. Bootstrap values of nodes exceeded 90%. Average intra\|species divergences were 0-0.0536 whereas inter\|specific divergences were 0.0554-0.1444. DNA barcoding analysis revealed that different aphid species live on different parts of P. armandii. Competition between aphid species has lead to differentiation in feeding sites. A combination of DNA barcoding and morphometry not only quickly distinguished different aphid species on P. armandii, but also detected morphologically cryptic species. Despite the lack of data in the species’ database, rapid identification is an effective method to resolve the identification of a large number of specimens in a short time.