Automated programming of degenerate primers and the cloning of the diamondback esterase gene.
Author of the article:HUANG Jing，WANG Shao-Li，QIAO Chuan-Ling
Author's Workplace：(Institute of Zoology, Chinese Academy of Sciences, the State Key Laboratory of Integrated Management of Pest Insects and Rodents,Beijing 100080, China).
Key Words：degenerate primer design; CodeHop insect esterase gene; RT-PCR
Abstract：It is a normal strategy to clone protein family cDNA using the degeneracy of the code to design PCR primers from protein multiple-sequence alignments. With the tools of blastp, blockmaker, CodeHop， SwissProt，and SpTrEMBL, degenerate primers were designe