Surface plasmon resonance (SPR) technique-A method to detect proteins' specific binding interactions.
Author of the article:CHEN Ju-Lian
Author's Workplace:(Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)
Key Words:surface plasmon resonance technology; evanescent wave biosensor; proteins binding interaction; insect signal transduction
Abstract:Surface plasmon resonance technique is a physical method to measure the special binding affinities of biological macromolecules. This article reviews SPR system characterization, experimental methods, optical configuration and detection principles, data c