A method of rapid preparation of trace-DNA templates of insects for PCR.
Author of the article:WEN Shuo-Yang1,2,HE Xiao-Fang1
Author's Workplace:(1.Department of Entomology, South China Agricultural University, Guangzhou 510642, China; 2.State Key Laboratory for Biocontrol Zhongshan University, Guangzhou 510275, China)
Key Words:trace-DNA templet; rapid extracted method; PCR
Abstract:Preparation of DNA templates from insect samples is a crucial prerequisite for successful PCR reactions. The classical method of phenolchloroform treatments for extracting DNA is complex and can not extract DNA from trace samples. A rapid, easy method f