Comparison of studies on Cry1Ac-binding in resistant and susceptible cells in Trichoplusia ni BTI-Tn-5B1.
Author of the article:LIU Kai-Yu,JIANG Cai-Fu,HONG Hua-Zhu,PENG Jian-Xin,YU Ze-Hua
Author's Workplace:(Institute of Entomology, Central China Normal University,Wuhan 430079,China).
Key Words:Bt Cry1Ac toxin; BTI-Tn-5B1 cell; binding peptide; resistance mechanism
Abstract:The selected-resistance ratio to Cry1Ac toxin in BTI-Tn-5B1 reached 1?280 after 56 generations. ELISA showed that the quantity of Cry1Ac toxin bound by resistant cells was reduced. Western bolt analysis revealed that there were five different molecular we