Cloning of the esterase gene in Plutella xylostella.
Author of the article:SHEN Ben-Chang1,ZHAO De-Xiu2,QIAO Chuan-Ling1
Author's Workplace:(1.The State Key Laboratory of I ntegrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China; 2. Institute of Botany, Chines e Academy of Sciences, Beijing 100094, China)
Key Words:Plutella xylostella L.; organophosphate pestici des; esterase; Cdna; Cdna library screening; RT-PCR
Abstract:RT-PCR was carried out with a pair of degenerate primers designed according to the reserved sequences of esterases in insects. A fragment of 330 bp was obtaine d, a BLAST search indicated that 10 different esterase genes were isolated, and spec I fic pr