Preparation and identification of the polyclonal antibodies from recombinant cat hepsin B.
Author of the article:AN Xiao-Meng,SHAO Hong-Lian,DU Xin-Jun,ZHAO Xiao-Fan,WANG Jin-Xing
Author's Workplace:(School of life Sciences, Shandong University,Jinan 250100 ,China)
Key Words:cathepsin B; preparation of antibody; immunoblottin g
Abstract:A method of preparation of polyclonal antibodies by immunizing rabbit w ith recombinant expressed cathepsin Blike proteinase (HCB) was introduced here . The Escherichia coli BL21,which harbored an expression vector of Pgex 4T-1/HC B was induced