Using capillary gas chromatography detect the activity of O-demethylation of cytochrome P450 in Helicoverpa armigera.
Author of the article:LIU Xiao-Ning,SHI Xue-Yan,LIANG Pei,GAO Xi-Wu
Author's Workplace:(Department of Entomology, China Agricultural University, Beijing 100094,China)
Key Words:capillary gas chromatogram; O-demethylation of cytochrome P450; Helicoverpa armigera
Abstract:A 875μL of reaction system determining the activity of p-nitroanisole O-demethylation by cytochrome p450 was developed based on the traits of the capillary gas chromatography(CGC), which has higher sensitivity, less sample volume and higher resolu