Construction of the cDNA library of Spodoptera exigua.
Author of the article:SUN Xiao-Jie1,2,LI Zhao-Fei1,YU Hang1,QIU Xue-Mei1,LI Qing2,ZHANG Wen-Qing1
Author's Workplace:(1.State Key Laboratory of Biocontrol, Sun Yatsen University, Guangzhou 510275; 2.Sichuan Agricultural University, Ya'an 625014)
Key Words:Spodoptera exigua; cDNA library; chitin synthase I
Abstract:A cDNA library of Spodoptera exigua(Hübner) was constructed by using a cDNA synthesis kit. The primary titre of the library was 1.1×105 pfu/mL, and the rate of recombination was 97%. The titre of amplified library was 5.4×107