迷迭香植物精油对白纹伊蚊的驱避作用及其化学成分
Repellent activities of essential oil from Rosmarinus officinalis on Aedes albopictus and its chemical constituents
李 黎1, 2，范泉水1，邱 薇1，张富强1，冯子良1，王 杰1，余 静1**， 刘祥义3*
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DOI：
作者单位：1.成都军区疾病预防控制中心博士后工作站 昆明 650032； 2.成都军区昆明总医院 昆明 650032；3.西南林学院基础部 昆明 650224
中文关键词：迷迭香，精油，气相色谱-质谱(GC/MS)，白纹伊蚊，驱避
英文关键词：Rosmarinus officinalis, essential oil, GC/MS, Aedes albopictus, repellence
中文摘要：
应用水蒸气蒸溜法和超临界二氧化碳萃取法提取生长于云南的迷迭香茎叶植物精油，测试2种精油对白纹伊蚊Aedes albopictus Skuse的驱避作用，并利用GC/MS联用仪分析2种精油的化学成分。结果表明：超临界二氧化碳提取的迷迭香精油对白纹伊蚊的驱避时间是（5.82±1.13) h，达到国标驱避剂评价的B级标准。2种精油化学成分中都含有驱纹活性物质：柠檬烯、樟脑、Eucalyptol、(+)-4-Carene、1-methyl-4-(1-methylethylidene)-cyclohexene、Beta-pinene等，且驱蚊活性成分在挥发油中占有较高比例，显示该植物精油在驱蚊方面有一定开发价值。
英文摘要： The effectiveness of essential oils from the stems and leaves of Rosmarinus officinalis L. as repellents of Aedes albopictus Skuse mosquito was assessed. Essential oils were extracted by steam distillation and supercritical CO2 extraction from plants growing in Yunnan Province and their chemical constituents isolated and identified using GC/MS methods. The results show that essential oils extracted by supercritical CO2 extraction were effective for sufficiently long (5.82±1.13) h to meet the public health insecticide B-standard for repellents. The active chemicals were (+)-Limonene、D-(+)-camphor、Eucalyptol、(+)-4-Carene、1-methyl-4-(1-methylethylidene)-cyclohexene. Beta-pinene were the major constituent of the essential oils extracted by either method. The results show that R. officinalis has potential as a commercial source of mosquito repellent.