刺槐叶瘿蚊越冬幼虫空间格局及抽样调查技术
Over-wintering larvae distribution pattern and sampling techniques of Obolodiplosis robiniae
高素红1,路常宽1** ,赵春明1 ,王晓勤1 ,曹晓亚1,宋国涛2,赵志波2
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作者单位：.河北科技师范学院农林有害生物监测技术研究所 秦皇岛 066600；2.昌黎县国营团林林场 昌黎 066600
中文关键词：刺槐叶瘿蚊，空间格局，抽样技术，越冬幼虫
英文关键词：Obolodiplosis robiniae， spatial distribution pattern， sampling techniques， over-wintering larvae
中文摘要： 用聚集指标法分析了刺槐叶瘿蚊Obolodiplosis robiniae (Haldemann)越冬幼虫的空间分布格局，结果表明：刺槐叶瘿蚊越冬幼虫在林间呈聚集分布；其聚集的原因与刺槐叶瘿蚊自身的行为习性有关或由刺槐叶瘿蚊本身与环境的异质性共同作用所造成。采用“z”字型、平行线、棋盘式、大五点、对角线5种抽样方法估计林间刺槐叶瘿蚊越冬幼虫虫口密度，确定对角线法调查15样方为最佳抽样方法。对土壤不同深度土层中越冬幼虫数量调查，明确最适取样深度为3 cm。同时利用Iwao回归建立了理论抽样数量模型：n=t2/D2(1.1957/+0.8905)。利用过筛手检和过筛水漂2种方法检数土中幼虫，对检出刺槐叶瘿蚊越冬幼虫虫量及检虫时间进行了对比分析，结果表明过筛水漂法检虫较为可取。
英文摘要： Five aggregation degree indices, the Taylor method and the Iwao method, were used to test the spatial distribution pattern of Obolodiplosis robiniae (Haldemann) in fields. The results indicate that O. robiniae had an aggregated distribution due to innate behavioral and environment factors. Over\|wintering larval population density was measured by 5 sampling methods; “Z” shape, parallel lines, ‘checkerboard’, twin\|diagonal lines and ‘big five points’, and 5, 10 and 15 samples obtained by each method compared through simulating sampling. The results show that collecting 15 samples using the twin\|diagonal lines method was the best sampling regime. 3cm proved a feasible soil depth to collect larvae. A theoretical sampling model n=t2/D2 (1.1957/ +0.8905) was developed. A comparison of the efficiency of collecting larvae from soil by hand and rinsing them from soil with water indicated that rinsing was superior.