外来入侵种红脂大小蠹COⅠ基因分化的研究
Differentiation of COⅠgene of the exotic red turpentine beetle Dendroctonus valens
姚 剑1**,温劲松1, 张龙娃2 ,李云飞1,陈雪娇1,余晓峰1
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作者单位：1.安徽出入境检验检疫局 合肥 230022; 2.安徽农业大学 合肥 230036
中文关键词：红脂大小蠹;COⅠ基因; 遗传分化;系统发育分析
英文关键词： Dendroctonus valens, COⅠgene, genetic differentiation, phylogenetic analysis
中文摘要： 红脂大小蠹Dendroctonus valens LeConte为近年来危害最为严重的外来入侵生物。本研究利用特异引物扩增出红脂大小蠹COⅠ基因序列 (GenBank登录号: GQ495096-GQ495128),在160个位点中发现3个该种与大小蠹属其他种不同的特异位点；比较不同地理种群的红脂大小蠹，发现中国种群间遗传分化不明显，说明入侵种的瓶颈效应，而遗传变异是入侵种与环境长期互作的结果；基于COⅠ基因序列比对的研究发现，入侵中国的红脂大小蠹与该虫原发生地北美洲部分种群具有同源性。
英文摘要： The red turpentine beetle, Dendroctonus valens LeConte (Coleoptera: Scolytidae), is one of the most dangerous invasive insect species in China. The sequences of its mitochondrial cytochrome oxidase subunitⅠ(COⅠ) were determined (GenBank Accession No. GQ495096-GQ495128). Sequence analysis revealed 3 specific loci among 160 genetic codes that distinguish D. valens from other Dendroctonus species. Comparison of different geographic populations indicates that genetic differentiation in Chinese populations of D. valens is not obvious, suggesting that genetic variation in local populations of this invasive species will require a long period of time under different local conditions. By aligning the COⅠgene sequences, we found that the sequences of red turpentine beetles in China were homologous to those from parts of the United States and spread to China with the increase in the international trade in timber.