一种烟粉虱成虫唾液酶鉴定与活性分析的方法
A method to identify and analyze salivary enzymes of Bemisia tabaci adults
彭 露1,2** ,严 盈2,万方浩2***, 王进军1
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作者单位：1.西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室 重庆 400716;2.中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室 北京 100081
中文关键词：刺吸式昆虫, 烟粉虱, 唾液酶, 鉴定与分析
英文关键词： piercing-sucking insect, Bemisia tabaci, saliva collection, identification and analysis
中文摘要： 以B型烟粉虱Bemisia tabaci (Gennadius)成虫为材料，介绍了一种微型刺吸式昆虫唾液酶鉴定和分析的方法，主要包括人工饲养、唾液收集、唾液多酚氧化酶（PPO）和过氧化物酶（POD）的鉴定与活性分析。结果显示，B型烟粉虱在特异性嗜好寄主甘蓝上分泌的多酚氧化酶与过氧化物酶的比活力分别为嗜好寄主番茄上的1.54和 1.65倍。该方法操作简捷，鉴定结果直观清晰，酶活测定灵敏，适合于其他微型刺吸式昆虫如蚜虫、木虱等的唾液酶研究。
英文摘要： A method to identify and analyze the salivary enzymes of small piercing-sucking insects, including artificial feeding, saliva collection, enzyme identification and analysis of salivary polyphenol oxidase and peroxidase, was developed using Bemisia tabaci (Gennadius) B\|biotype adults. The result show that PPO and POD activity in the watery saliva of B. tabaci B\|biotype feeding on cabbage were respectively 1.54 and 1.65 fold higher than those feeding on tomato. This new method provides a simple intuitive and sensitive way of identifying and measuring the activity of enzymes in insect saliva. This method can also be applied to other small piercing-sucking insects, such as aphids and psyllids.