云南西花蓟马rDNA-ITS2遗传多态性及其种群扩张
Genetic diversity and population expansion in different geographic populations of Frankliniella occidentalis in Yunnan， China
沈登荣1，2**,张宏瑞1，2,李正跃1，2 ,董坤1，3,和绍禹1，3***
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作者单位：1云南农业大学农业生物多样性与病虫害控制教育部重点实验室昆明650201；2云南农业大学植物保护学院昆明650201；3云南农业大学东方蜜蜂研究所昆明650201
中文关键词：西花蓟马，遗传分化，基因流，种群扩张
英文关键词：Frankliniella occidentalis， genetic differentiation， gene flow， population expansion
中文摘要： 应用rDNA-ITS2基因序列对云南各地理种群西花蓟马Frankliniella occidentalis （Pergande）的遗传结构和遗传分化程度进行初步研究。经过比对112条序列，共发现了59个变异位点，定义了30种单倍型。云南省西花蓟马的单倍型多态性较高（Hd=0.90219），而核酸多态性较低（Pi=0.00891）。各地理种群西花蓟马的遗传分化指数Fst为0.00810，基因流Nm为30.61，表明各地理种群间遗传分化程度非常低，种群间存在充分的基因交流。对群体进行中性检验、错配分析表明西花蓟马群体曾经历过近期的种群扩张。分子方差分析（AMOVA）表明，云南西花蓟马的遗传变异主要来自于种群内部，种群间的遗传变异水平还非常低。从分子生物学的角度上也证实了西花蓟马近期入侵云南的事实。
英文摘要： The rDNA-ITS2 gene was used to investigate genetic structure and differentiation among populations of Pergande, Frankliniella occidentalis（Pergande） , in Yunnan Province. 112 rDNA-ITS2 sequences were analyzed, including 59 variable sites, defining 30 haplotypes. Haplotype diversity was quite high (Hd=0.90219) overall but nucleotide diversity was low (Pi=0.00891). The Fst value was 0.00809 and gene flow was Nm=30.64 among all populations. These results suggest that genetic differentiation was low and that gene flow between different geographic populations was sufficient. Neutral test and mismatch analysis indicate that F. occidentalis has undergone a recent population expansion. The results of AMOVA also suggest that observed genetic differences mainly originate from interpopulation differences, with relative little genetic variation within populations. These molecular data are consistent with the recent invasion of Yunnan by F. occidentalis.