西花蓟马对多杀菌素的抗性汰选和遗传方式
Laboratory selection and inheritance mode of resistance to spinosad in the western flower thrips，Frankliniella occidentalis
王泽华**,吴青君***,徐宝云,张友军
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作者单位：中国农业科学院蔬菜花卉研究所北京100081
中文关键词：西花蓟马，多杀菌素，抗性汰选，抗性遗传
英文关键词： Frankliniella occidentalis，spinosad， laboratory selection， resistance inheritance
中文摘要： 在室内进行了西花蓟马Frankliniella occidentalis （Pergande）种群对多杀菌素的抗性筛选和抗性遗传方式分析。经过2年多的汰选，抗性水平相比较初始种群提高了30倍，与同时测定的敏感品系相比抗性达到80.8倍。抗性遗传方式分析结果表明，正、反交后代显性度分别为0.51和0.43，二者差异不显著，说明西花蓟马对多杀菌素的抗性为常染色体、不完全显性遗传；剂量对数-死亡机率值曲线分析结果显示回交后代在死亡率50%处，自交后代在死亡率25%和75%处未出现明显平坡，回交和自交后代实测值卡方检验进一步证实抗性为多基因控制。
英文摘要： The response of western flower thrips, Frankliniella occidentalis （Pergande）, to selection for resistance to spinosad under laboratory conditions, and the mode of inheritance of spinosad resistance was investigated.After more than 2 years of selection resistance to spinosad in the selected strain increased 30-fold compared to the original strain, and 8.8-fold compared to the susceptible strain.The progeny of reciprocal F1 crosses (resistant male×susceptible female and resistant female×susceptible male) responded similarly in bioassays, with values of 0.51 and 0.42 for D（degree of dominance）, respectively, indicating that resistance was autosomal and incompletely dominant.Analysis of dose-response lines indicates that there were no plateaus at 50% mortality of backcross progeny (F1×susceptible strain) and at 25% and 75% mortalities of self-cross of backcross progeny.Chi-square analysis of the resistance of progeny from backcrosses (F1×susceptible strain) and self-cross of backcrosses suggest that resistance to spinosad is probably controlled by several genes.