（中国农业大学农学与生物技术学院北京100193）摘要为探明绿盲蝽Apolygus lucorum（MayerDür) 取食和机械损伤对不同抗性棉花叶片内主要防御酶活性的影响以及防御酶与棉花抗绿盲蝽性的关系，以棉花3个不同抗性品系为材料，室内条件下测定绿盲蝽取食和机械损伤处理后棉叶中苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）的活性。结果表明：对于健康植株，抗性品种PAL、POD和PPO的活性均显著高于感性品种防御酶的活性；在绿盲蝽取食诱导条件下，不同抗性棉叶的PAL、POD和PPO活性增加，且抗性品种比感性品种达到最大值的时间早；在机械损伤处理后，不论是抗性品种还是感性品种，PAL、POD和PPO活性变化都不及绿盲蝽取食诱导明显。本研究说明，棉叶中PAL、POD和PPO活性对棉花的抗绿盲蝽性有一定的影响，这些酶活性的变化与逆境条件、胁迫时间以及棉花的品种有关。
关键词绿盲蝽, 棉花, 取食, 机械损伤, 防御酶活性

Effects of Apolygus lucorum feeding and mechanical damage on defense
enzyme activities in cotton leaves MAO Hong**CHEN HanLIU XiaoXiaZHANG QingWen***
(College of Agriculture and Biotechnology, China Agricultural University, Beijing100193, China)

AbstractActive changes in the levels of the defensive enzymes phenylalanine aminolyase（PAL), peroxidase（POD) and polyphenol oxidase（PPO), were evaluated in the leaves of various cotton varieties that had either been fed on by Apolygus lucorum (MayerDür), or subject to mechanical damage, under laboratory conditions. The results indicate that PAL, POD and PPO activity were significantly higher in resistant than in susceptible varieties. PAL, POD and PPO activity increased in each variety following damage by A. lucorum but peaked in resistant varieties earlier than in susceptible varieties. However, changes in PAL, POD and PPO activity following mechanical damage were not as obvious as those observed following feeding by A. lucorum. These results suggest that PAL, POD and PPO activities are associated with the resistance of cotton to A. lucorum and that changes in defensive enzyme activity depend on the cotton variety and the kind of damage plants are subject to.
Key wordsApolygus lucorum, cotton, feeding, mechanical damage, defense enzyme activity