转双价基因(Bt+ CpTI)棉对棉铃虫中肠羧酸酯酶活性的诱导作用
Effects of transgenic Cry1Ac + CpTI cotton on carboxylesteraseactivity in Helicoverpa armigera
李秀霞 范银君 亓永凤 宋敦伦 高希武 等
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作者单位：中国农业大学农学与生物技术学院北京100193
中文关键词：棉铃虫，羧酸酯酶，转基因棉花
英文关键词：cotton bollworm，CarE，transgenic cotton
中文摘要： 为了明确棉铃虫Helicoverpa armigera(Hübner)取食转双价基因( Bt + CpTI)棉叶以及取食一段时间转基
因棉叶后，再取食常规棉叶，对棉铃虫取食量、体重增长量及中肠羧酸酯酶活性的影响，本研究分别用转双价基因
棉叶和常规棉叶饲喂4 龄棉铃虫幼虫5 d，比较考察了棉铃虫的取食量、体重增长量及中肠羧酸酯酶的活性;另外
分别考察了棉铃虫取食转基因棉叶1 d 及3 d 后再取食常规棉叶，其中肠羧酸酯酶的变化。结果表明，持续取食
常规棉花叶片5 d 的棉铃虫，其中肠羧酸酯酶的活性持续升高;而持续取食转基因棉叶5 d 的棉铃虫，其中肠羧酸
酯酶的活性先升高后降低。取食转基因棉花叶片1 d 后，取食常规棉叶的棉铃虫，其中肠羧酸酯酶的活性，随着换
取常规棉叶时间的延长，活性逐渐降低;而棉铃虫在取食3 d 转基因棉叶后再取食常规棉叶，其中肠羧酸酯酶活性
却一直保持较高。可见，棉铃虫在取食转基因棉花后，其羧酸酯酶活性可以被诱导，这应与棉铃虫对转基因棉的
抗性及防御性有一定关系。
英文摘要：In order to clarify changes in the carboxylesterase ( CarEs) activity of Helicoverpa armigera( Hübner) feeding
on transgenic cotton，and the recovery of carboxylesterase activity in H． armigera that fed on non-transgenic cotton after
feeding on transgenic cotton for 1 to 3 days，transgenic and non transgenic cotton leaves were fed to some fourth-instar H．
armigera larva for 5 days，after which some were fed with non-transgenic cotton leaves for a few days after feeding on
transgenic cotton leaves for 1 day or 3 days． Midgut CarEs activity was examined every 24 hours． The results show that
CarEs activity in H． armigera fed on non-transgenic cotton leaves increased over time，whereas that of H． armigera fed on
transgenic cotton leaves first increased then decreased． The enzyme activity of cotton bollworms fed on non-transgenic
cotton leaves after feeding on transgenic cotton leaves for one day decreased gradually over time，whereas that of those fed
on non-transgenic cotton leaves after feeding on transgenic cotton leaves for three days remained higher than the control．
These results indicate that CarEs activity in H． armigera can be induced by feeding on trangenetic cotton leaves and that
this plays a role in the resistance of H． armigera to the Cry1Ac toxin．