蜂群中不同亚家庭的工蜂寿命分析
Analysis of lifespans of workers from different subfamiliesin a honeybee colony
王欢**,张少吾,张飞,曾志将***
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DOI：
作者单位：江西农业大学蜜蜂研究所南昌330045
中文关键词：工蜂，亚家庭，微卫星，寿命，生存分析
英文关键词：worker，subfamily，microsatellite，lifespan，survival analysis
中文摘要：在蜂群中，蜂王与多个雄蜂自然交尾，形成不同亚家庭。为了研究不同亚家庭中工蜂寿命是否有差异，
我们以西方蜜蜂Apis mellifera L． 为实验材料，随机从一群自然群中取270 只刚孵化的工蜂，单独饲养于有蜜粉脾
小蜂箱内，每天将自然死亡个体取出标记。并利用3 对微卫星进行个体基因型分析，通过Matesoft 软件划分亚家
庭，然后分析了各亚家庭工蜂的自然寿命及生存曲线。结果表明:实验蜂群由12 个亚家庭组成，其中2 个亚家庭
工蜂寿命与其他亚家庭存在显著差异(P ＜ 0. 05)。
英文摘要：In colonies，virgin queens naturally mate with several males，forming subfamilies． In order to find differences
in lifespan between subfamilies，an experiment was conducted with Apis mellifera L． We collected 270 emerging workers
from a naturally mated queen colony，holding them separately in a small box with a comb with sufficient food，and picked
out and counted the naturally dead insects every day． We determined genotypes of marker workers based on 3
microsatellite primers and identified the patrilines． Survival curves of the different subfamilies were drawn for survival
analysis． The results indicate that the experimental colony was made up of 12 subfamilies，with two of these having
significant survival differences from the other subfamilies (P ＜ 0. 05) ．