三叶蔓荆子提取物对小菜蛾的拒食活性
Antifeeding activity of Vitex trifolia extracts against third-instar larvae of Plutella xylostella
高燕1，盛广为2，沈嘉祥2，肖春2**
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DOI：
作者单位：1. 广东省农业科学院植物保护研究所广州510640； 2. 云南农业大学植物保护学院昆明650201
中文关键词：三叶蔓荆子，小菜蛾，拒食活性
英文关键词：Vitex trifolia，Plutella xylostella，antifeeding activity
中文摘要：室内测定了三叶蔓荆子( Vitex trifolia (L． ))叶片提取物对小菜蛾 Plutella xylostella(L． ) 3龄幼虫的拒食活性。非选择性试验结果表明，三叶蔓荆子的不同溶剂 (乙酸乙酯、石油醚、氯仿和乙醇 )提取物对小菜蛾均有一定的拒食作用。4种提取物对小菜蛾的毒力顺序为:乙酸乙酯提取物＞石油醚提取物＞氯仿提取物＞乙醇提取物。选择性试验表明，乙酸乙酯提取物对小菜蛾 24、48 h的拒食中浓度 ( AFC50 )分别为 2 520、3 240 mg·L－1。室内盆栽试验结果表明，施用乙酸乙酯提取物(10 000 mg·L－1 ) 3d后甘蓝 ( Brassica oleraceavar． capitata)植株上 3龄小菜蛾的虫口减退率可达 72. 76%.
英文摘要：
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The anti feeding activity of extracts of Vitex trifolia (L． ) leaves against the 3rd instar larvae of Plutella xylostella (L． ) was tested in alaboratory． A no-choice experiment revealed that extracts extracted with either ethyl acetate，petroleum，ether or chloroform had significant antifeedingactivity． The relative antifeeding activity of extracts obtained with each of these four solvents was，in descending order; ethyl acetate＞ petroleum ether＞ chloroform＞ethanol． A choice-experiment indicated that the AFC50 of the ethyl acetate extract was 2 520 mg·L－1 24 h after treatment
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and3240 mg·L－1after 48 h． The number ofP． xylostella larvae on Brassica oleraceavar． capitata plants could be reduced by 72. 76%three days after spraying with ethyl acetate extract．