准噶尔小胸鳖甲抗冻蛋白MPAFP5毕赤酵母表达产物的理化性
Characterization of antifreeze protein MPAFP5 from Microdera punctipennis
dzhungarica expressed in Pichia pastoris.
吕国栋;孙洁;马纪;张富春
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作者单位：新疆大学生命科学与技术学院分子生物学重点实验室新疆生物资源基因工程重点实验室
乌鲁木齐830046
中文关键词：准噶尔小胸鳖甲，抗冻蛋白，理化性质，等电点
英文关键词：Microdera punctipennis dzhungarica; antifreeze pr
otein; physical-chemical properties; isoelectric focusing point
中文摘要：研究准噶尔小胸鳖甲Microdera punctipennis dzhungarica Kasz抗冻蛋白MPAFP5真核表达产物的功能及理化性质。首先用硫酸铵浓度梯度沉淀初步纯化酵母真核表达产物，然后通过细菌低温保护实验检测抗冻蛋白MPAFP5低温保护功能,同时探讨抗冻蛋白MPAFP
英文摘要：In order to test the function and the physicalchemical properties of the eukaryotic products of antifreeze protein gene mpafp5 cloned from Microdera punctipennis dzhungarica Kasz, the isoelectric point of MPAFP5 was determined by MI