黑翅土白蚁踪迹信息素组分数量的柱层析分离和活性测定分析
Liquid chromatography and bioassay analysis of components in the trail pheromone of the blackwing subterranean termite Odontotermes formosanus Shiraki
文平1,3**，嵇保中1***，刘曙雯2，冀仕琳1，刘佳佳1
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作者单位：1. 南京林业大学森林资源与环境学院南京210037； 2. 南京中山陵园管理局南京210014；3. 江苏省农用激素工程技术研究中心常州213022
中文关键词：黑翅土白蚁， 踪迹信息素， 柱层析， 活性组分
英文关键词：Odontotermes formosanus, trail pheromone, liquid chromatograph, active components
中文摘要：
本文分析了在活性限浓度进行生物活性测定时，黑翅土白蚁Odontotermes formosanu Shiraki对踪迹信息素的行为反应特点以及使用不同踪迹线载体对踪迹信息素活性的影响。并设计标准生物活性测试方法，测定了采用柱层析方法分离的黑翅土白蚁踪迹信息素各分离馏分的活性，发现黑翅土白蚁踪迹信息素用22%乙酸乙酯/正己烷洗脱液等度洗脱分离后的各馏分中包含两个强活性区段，推测黑翅土白蚁踪迹信息素至少包含2个有轻微极性差异的组分。
英文摘要：By working at the threshold active concentration, the characteristic behavioral reactions of the blackwinged subterranean termite Odontotermes formosanus Shiraki to extracts of trail pheromone components were analyzed in trailfollowing bioassays, including the effect of different testing substrates. Based on the results, a standard bioassay method was used to test the fractions from isocratic elution (22% EtOAc/nhexane) of pheromone extract on a silica gel column. Results show that two active peaks exist in the fractions, which indicate that the trail pheromone of O. formosanus consists of at least two components.