中华蜜蜂哺育蜂与采集蜂行为转变相关基因的表达差异研究
Analysis of differentially expressed genes associated with the behavioral transition between nurses andforagers in Apis cerana cerana
宗 超** 刘 芳 余林生*** 汪天澍 施腾飞
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作者单位：安徽农业大学动物科技学院 合肥 230036
中文关键词：哺育蜂，采集蜂，实时荧光定量PCR，表达差异
英文关键词：nurses, foragers, Real-time Quantitative Reverse Transcription Polymerase Chain, differential expression
中文摘要： 【目的】 文章旨在寻找中华蜜蜂Apis cerana cerana哺育蜂与采集蜂行为转变相关的重要基因。【方法】 选取了8群中华蜜蜂，分别采集哺育蜂和采集蜂，然后通过实时荧光定量PCR对哺育蜂和采集蜂头部中mrjps、Ache、CSP3等22个基因的表达进行了分析。【结果】 实验结果表明：mrjp3、mrjp7、Ache、LOC406142、Hbg3、Mblk-1、Ef-1a-f1、TpnCIIIa、Wat、Ant基因在哺育蜂和采集蜂中表达差异极显著（P<0.01），mrjp1、mrjp2、mrjp4、LOC406114、CSP3、Dop1、Jhe、Oa1、Per、TpnT基因在哺育蜂和采集蜂中表达差异显著（0.01<P<0.05），mrjp6、obp3基因在哺育蜂和采集蜂中表达差异不显著（P >0.05）。【结论】 mrjp3、mrjp7、Ache、LOC406142、Hbg3、Mblk-1、Ef-1a-f1、TpnCIIIa、Wat、Ant、mrjp1、mrjp2、mrjp4、LOC406114、CSP3、Dop1、Jhe、Oa1、Per、TpnT 这些表达差异显著的基因很可能与中华蜜蜂哺育蜂与采集蜂的行为转变有关。本研究的结果为我们更好地认识理解蜜蜂行为转变的机制提供思路。
英文摘要： [Objectives] To find important genes associated with the behavioral transition between nurses and foragers in the honeybee Apis cerana cerana. [Methods] Total RNA was extracted from nurses and foragers of eight colonies of A. cerana cerana. Real-time Quantitative Reverse Transcription Polymerase Chain (RT-qPCR) was used to detect differences in genes between nurses and foragers. [Results] The results revealed that the expression of the mrjp3、mrjp7、Ache、LOC406142、Hbg3、Mblk-1、Ef-1a-f1、TpnCIIIa、Wat and Ant genes were significantly different between nurses and foragers (P<0.01). The mrjp1、mrjp2、mrjp4、LOC406114、CSP3、Dop1、Jhe、Oa1、Per and TpnT were also differentially expressed between nurses and foragers but the difference was less significant (P<0.05). The mrjp6 and obp3 genes were not differentially expressed (P>0.05). [Conclusions] Differentially expressed genes may play an important role in the behavioral transition between nurses and foragers and identifying such genes may improve our understanding of the mechanism of behavioral transition in worker bees.