蝗虫微孢子虫Nosema locustae 对红胫戟纹蝗Dociostaurus kraussi kraussi 致病性及呼吸代谢的影响
Pathogenicity of Nosema locustae to Dociostaurus kraussi kraussi
扈鸿霞1** 王 晗1 石旺鹏2 于 非1 王志勇1 季 荣1***
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作者单位：1新疆师范大学生命科学学院，中亚区域动物资源保护及有害生物控制实验室，乌鲁木齐 830054；2.中国农业大学农学与生物技术学院，北京 100000
中文关键词：蝗虫微孢子虫，红胫戟纹蝗，致病性，呼吸代谢
英文关键词： Nosema locustae, Dociostaurus kraussi kraussi, pathogenicity, respiratory metabolism
中文摘要：
【目的】 研究蝗虫微孢子虫Nosema locustae对红胫戟纹蝗Dociostaurus kraussi kraussi致病性及呼吸代谢的影响，为筛选蝗虫微孢子虫适宜的新疆本地的活体增殖寄主提供实验依据。【方法】 采用逐头口服法感染红胫戟纹蝗，以镜检法检测蝗虫感染情况，并用呼吸仪测量试虫的呼吸代谢。【结果】 在6.5×105 个孢子/头的感染剂量下，红胫戟纹蝗的感染率和死亡率分别为41.70%和72.22%。蝗虫微孢子虫的感染剂量与其毒力高度相关（r2=0.961），LD50为1.7088×104 个孢子/头；随孢子浓度增加和感染时间延长，试虫的CO2释放率显著降低（P<0.05），但耗氧率没有显著变化。【结论】 红胫戟纹蝗被微孢子虫感染后表现出典型症状，高剂量组感染下死亡率达到70%以上，由此推断红胫戟纹蝗可作为微孢子虫在新疆本地的潜在增殖寄主。
英文摘要： [Objectives] To study the pathogenicity of Nosema locustae to Dociostaurus kraussi kraussi, and to provide an experimental basis for screening living hosts of N. locustae in Xinjiang. [Methods] D. kraussi kraussi were orally infected with Nosema locustae and the virulence and effects of fungal infection on the host respiratory metabolism were determined. [Results] The results show that, at a dose of 6.5×105 spores, the infection and mortality of D. kraussi kraussi were 41.7% and 72.22%, respectively. There was good correlation between infective dose and virulence of N. locustae (r2=0.961), the LD50 was 1.7088×104 spores. Compared to the control, increasing spore concentration and infection time significantly decreased CO2 emissions (P<0.05). [Conclusion] N. locustae can infect D. kraussi kraussi with high lethality and has significant effects on the hosts’ respiratory metabolism.