亚致死剂量毒死蜱对飞蝗细胞色素P450酶活性及基因表达的影响
Effect of sub-lethal doses of chlorpyrifos on cytochrome P450 enzyme activity and gene expression in Locusta migratoria
郭艳琼1, 2** 张建珍1 郭亚平1 马恩波1***
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作者单位：1. 山西大学应用生物学研究所 太原 030006；2. 山西农业大学农学院 太谷 030801）
中文关键词：亚致死剂量，毒死蜱，飞蝗，细胞色素P450
英文关键词：sublethal doses, chlorpyrifos, Locusta migratoria, cytochrome P450
中文摘要： 【目的】 研究有机磷杀虫剂毒死蜱对飞蝗体内细胞色素P450的影响。【方法】 采用酶活力测定法和实时定量PCR技术分别研究了毒死蜱3种亚致死剂量（LD10、LD30和LD50）处理飞蝗3龄幼虫24 h后，体内细胞色素P450酶活性及CYP409A1和CYP408B1基因表达量的变化。【结果】 不同亚致死剂量毒死蜱处理引起细胞色素P450活性显著性降低，分别为对照组的0.68、0.50和0.62倍。同时通过mRNA水平表达的差异比较显示，飞蝗的两个P450基因CYP409A1和CYP408B1的表达受到抑制，均出现表达量减少的现象。【结论】 某些细胞色素P450基因表达受不同亚致死剂量毒死蜱的抑制而使酶的量被降低，从而造成飞蝗整体细胞色素P450酶活性的下降。
英文摘要： [Objectives] To investigate the effects of sub-lethal doses of chlorpyrifos (organophosphate) on cytochrome P450 activity in Locusta migratoria. [Methods] Three doses (LD10, LD30 and LD50) of chlorpyrifos were applied to specimens of L. migratoria and the cytochrome P450 monooxygenase activity of 7-EC-O-deethylation (ECOD) was measured and the mRNA levels of CYP409A1 and CYP408B1 were analyzed by Real-time RT-PCR after 24 h. [Results] ECOD activity decreased by 0.50-0.68-fold after chlorpyrifos exposure and mRNA expression of CYP409A1 and CYP408B1 also decreased. Conclusion] Chlorpyrifos can significantly inhibit cytochrome P450 enzyme activity by depressing the transcription of multiple cytochrome P450 genes.