基于ITS条形码序列的刺桐姬小蜂分子鉴定
Application of DNA barcoding technology to the identification of Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae)
黄蓬英1** 林玲玲1 李雯琳2 洪钦阳2 张玲华2 廖富荣
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作者单位：1. 厦门出入境检验检疫局 厦门 361026；2. 福建农林大学植物保护学院 福州 350003
中文关键词：刺桐姬小蜂，ITS基因，DNA条形码，特异引物，分子鉴定
英文关键词：Quadrastichus erythrinae, ITS gene, DNA barcoding, diagnostic primers, molecular identification
中文摘要： 【目的】 刺桐姬小蜂Quadrastichus erythrinae Kim体型小，传统的形态学鉴定方法难以快速准确识别。【方法】 本研究测定了刺桐姬小蜂的rDNA ITS1和ITS2序列，根据18S rDNA 部分序列，利用MEGA的最大相似法（Maximum Likehood）构建系统发育树。根据刺桐姬小蜂ITS1和ITS2序列设计了特异引物，应用特异引物对单只刺桐姬小蜂进行PCR扩增，可稳定地扩增出明显的目的DNA条带。【结果】 研究表明，基于ITS基因的DNA条形码技术可以用于刺桐姬小蜂的快速准确鉴定。【结论】 因此，采用ITS1和ITS2区的特异性引物可对刺桐姬小蜂进行快速分子鉴定。
英文摘要： [Objectives] Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae) is too small to be identified quickly and accurately by traditional morphological methods. [Methods] The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q. erythrinae. A phylogenetic tree was established on the basis of variation in 18SrDNA part sequences using MEGA Maximum Likelihood. Species-specific primers of Q. erythrinae were designed based on the rDNA ITS1 and ITS2 sequences. The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species. [Results] The results suggest that the species-specific primers developed produce clear, unique and reproducible target DNA bands from individual adult wasps. Molecular identification of Q. erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible. [Conclusion] This study identified a possible avenue for the molecular identification of Q. erythrinae.