一种深色型粘虫成虫体色突变体的遗传分析
Genetic analysis of a Mythimna separate (Walker) body color mutant
李 栋 张云慧 李祥瑞 程登发
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作者单位：中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室 100193
中文关键词：粘虫，体色突变体，遗传分析
英文关键词：Mythimna separata, body color mutant, genetic analysis
中文摘要：
【目的】 在粘虫Mythimna separate（Walker）常规饲养种群和室外监测中发现一种成虫体色突变体，本文主要研究其遗传模式及进行表型观察。【方法】 用杂交实验的方法对该突变体进行观察研究。【结果】 与正常型相比，该突变体的腹、翅颜色等形态特征与正常型有显著的不同；卵、幼虫的颜色与正常型的没有明显的区别；蛹的颜色前期没什么区别，临近羽化时表现为颜色较深。正常型与突变体正反交F1代均为正常型，F2代正常型和突变体分离比例为3.24︰1，回交比例为1.18︰1，F2代突变体自交，后代全为突变体。【结论】 该突变体的遗传符合孟德尔的遗传规律，属于常染色体上单基因控制的隐性遗传性状。本项研究结果可以为粘虫的遗传防治、进化上的环境适应性及内在分子机制研究提供参考。
英文摘要： [Objectives] Body color mutants of Mythimna separate (Walker) were found in a laboratory colony and in the wild and their phenotypes observed. [Methods] A hybridization experiment was used to analyze the genetic basis of this mutation. [Results] Compared with normal moths, the adult body of mutants, especially the abdomen and wings, were darker in color. There were no obvious differences in the appearance of eggs, larvae and early pupae, but immediately before eclosion, mutant pupae were darker in color than normal pupae. The results of the hybridization experiment show that the proportion of the normal type in F1 progeny of reciprocal crosses between normal and mutant parents was 100%, whereas the ratio of F2 segregation between normal and mutant types was 3.24︰1. The ratio in backcrosses was 1.18︰1. In addition, progenies of self-crossed F2 mutants were exclusively of mutant type. [Conclusion] The results of the hybridization experiment are consistent with Mendelian, monogenic autosomal recessive inheritance. These results shed light on the molecular mechanism determining moth body color differentiation at both the genetic and environmental levels and contribute to the theoretical basis for the prediction and integrated management of M. separata.