转cry1C基因抗虫稻谷对黄粉虫生长发育的影响
Effect of transgenic cry1C rice on the development of the yellow mealworm (Coleoptera: Tenebrionidae)
王加美1, 2** 葛东华1 陈秀萍1*** 丁家桐2 彭于发1
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DOI：10.7679/j.issn.2095-1353.2014.141
作者单位：1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193； 2. 扬州大学动物科学与技术学院，扬州 225009
中文关键词：转cry1C基因水稻，黄粉虫，非靶标效应，安全评价
英文关键词：transgenic cry1C rice, yellow mealworm, non-target effect, safety assessment
中文摘要：
【目的】 研究转cry1C基因抗虫水稻（T1C-19）稻谷对黄粉虫生长发育的影响，为转Bt基因水稻的推广和应用提供理论支持。【方法】 在试验室条件下，分别用100% T1C-19稻谷、100% 明恢63（MH63）稻谷、62% T1C-19稻谷、62% MH63稻谷和正常饲料饲喂黄粉虫幼虫直至其化蛹，比较各处理组体重、存活率、幼虫历期、化蛹率和羽化率等检测指标。【结果】 正常饲料组、62% T1C-19稻谷组和62% MH63稻谷组的黄粉虫幼虫体重几乎都显著高于100% T1C-19稻谷组和100% MH63稻谷组，但T1C-19和MH63组间差异不显著；此外，5组间的存活率、幼虫历期、化蛹率及羽化率差异均不显著。【结论】 黄粉虫可通过取食转cry1C基因稻谷暴露于Cry1C蛋白中，但黄粉虫的生长发育没有受到明显的负面影响；转cry1C基因稻谷可作为饲料原料用于黄粉虫养殖。
英文摘要：
[Objectives] The effects of transgenic cry1C rice (T1C-19) on the development of the yellow mealworm were assessed to provide useful information for the commercial cultivation of Bt rice. [Methods] Larvae of the yellow mealworm were randomly assigned to be fed with 100% T1C-19, 100% MH63, 62% T1C-19, 62% MH63 rice grain, or a normal diet, until they pupated and their body weight, survival rate, larval stage duration, pupation rate and eclosion rate were measured and analyzed. [Results] The body weights of the 62% T1C-19, 62% MH63 rice grain group and the normal diet group were almost significantly higher than those of 100% T1C-19 rice and 100% MH63 rice group, and there were no statistically significant differences between the T1C-19 group and MH63 group. Moreover, there were no significant differences in survival rate, duration of larval stage, pupation rate and eclosion rate among the above five different treatments. [Conclusion] Transgenic cry1C rice has no obvious adverse effects on the development of the yellow mealworm and can therefore be used as a feed for this species.