Q型烟粉虱为害对不同品种辣椒保护酶活性及次生物质含量的影响
Effects of Bemisia tabaci damage on the protective enzyme activity and the secondary metabolite content of leaves indifferent pepper varieties
孔海龙1** 吕 敏2 吴 琳1 祝树德1***
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DOI：10.7679/j.issn.2095-1353.2014.184
作者单位：1. 扬州大学园艺与植物保护学院，扬州 225009；2. 江苏里下河地区农科所，扬州 225009
中文关键词： 辣椒品种，Q型烟粉虱，成虫取食，SOD，POD，次生物质
英文关键词：pepper varieties, Q biotype Bemisia tabaci, adult feeding, SOD, POD, secondary metabolites
中文摘要：
【目的】 探明辣椒品种对烟粉虱Bemisia tabaci的抗性机制。【方法】 对Q型烟粉虱为害辣椒后不同抗性品种的保护酶活性和次生物质含量进行了测定。【结果】 Q型烟粉虱取食辣椒后，不同品种叶片SOD、POD活性及总酚、总黄酮含量存在显著差异。成虫取食后，6个品种辣椒的SOD活性均升高，除了感虫品种港上红尖椒外，其余5个品种的SOD活性均与对照存在显著差异。 抗虫品种（苏椒5号和新研988椒王）、中等抗虫品种（萧新九号和湘辛三号）的POD活性明显增强，而感虫品种(港上红尖椒和大禹牛角王)的POD活性明显降低。从SOD、POD活性变化值来看，抗虫品种最大，中等抗虫品种次之，敏感品种最小。Q型烟粉虱为害辣椒后，大多数品种叶片总酚和总黄酮含量均显著升高。总酚含量以抗虫品种苏椒5号明显高于其他品种。从总黄酮含量变化值来看，抗虫品种反而较敏感品种较小。【结论】 供试6个不同品种对Q型烟粉虱的抗性强弱与辣椒叶片SOD、POD活性、总酚含量有关，而与总黄酮含量无明显相关。
英文摘要：[Objectives] To investigate the mechanism underlying the resistance of pepper varieties to whitefly. [Methods] Protective enzyme activity and the secondary metabolite content were studied in the leaves of different varieties pepper plant damaged by the Q biotype whitefly. [Results] The results show that there was a significant difference in the activity of SOD and POD and the total phenolic content in the leaves of different pepper varieties fed on by Q the biotype whitefly. SOD activity increased after whitefly damage in all six varieties peppers. Except for the susceptible Gangshanghong pepper variety, SOD activity in the other five varieties was significantly different from the control, and POD activity of resistant varieties ( Sujiao 5 and Xinyan 988 pepper king ) and moderately resistant varieties (Xiaoxin 9 and Xiangxin 3) increased significantly relative to the control. In contrast, POD activity of the susceptible varietiesGangshanghongandDayuniujiaowang) were significantly less than that of the control. The difference in SOD and POD activity in resistant varieties between damaged and undamaged pepper leaves was highest, followed by the medium resistant varieties, whereas the least difference was observed in the susceptible varieties. Total leaf phenolic and total flavonoid content in most pepper varieties significantly increased after whitefly infestation, and the total phenolic content of the resistant variety Sujiao 5 was significantly higher than that of the other varieties. The change in total flavonoids in resistant varieties between damaged and undamaged leaves were less than those in susceptible varieties. [Conclusion] The resistance of six different pepper varieties against the Q biotype whitefly appears related to SOD and POD activity and total phenolic content, but not to the total flavonoid content, of pepper leaves.