观察球孢白僵菌侵染烟粉虱若虫过程的新方法——荧光显微法
A new fluorescent microscopy method for identifying Beauveria bassiana infected Bemisia tabaci nymphs
王登杰1** 雷仲仁1 王帅宇2 王海鸿1***
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DOI：10.7679/j.issn.2095-1353.2015.028
作者单位：1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京 100192 2. 北京市植物保护站，北京 100029
中文关键词：烟粉虱，若虫，白僵菌，荧光显微法
英文关键词： Bemisia tabaci, nymph, Beauveria bassiana, fluorescence microscopy method
中文摘要：
【目的】 介绍一种观察白僵菌Beauveria bassiana侵染烟粉虱Bemisia tabaci (Gennadius)若虫的荧光显微方法。【方法】 将被白僵菌侵染的烟粉虱若虫用荧光素二乙酸酯(FDA)染色，并于波长为450～490 nm的蓝光下显微观察。【结果】 在接种白僵菌12 h和24 h的烟粉虱若虫上分别观察到昆虫表皮上分生孢子的萌发和芽管穿透表皮。结合裸眼和荧光显微观察结果证实了被真菌侵染的烟粉虱若虫体色变红和菌体在虫体内增殖是同时发生的。【结论】 应用荧光显微方法能观察到白僵菌在烟粉虱若虫体表和体内的侵染。
英文摘要：[Objectives] To assess the effectiveness of a fluorescent microscopy method developed for identifying Bemisia tabaci (Gennadius) nymphs infected with the Beauveria bassiana fungi. [Methods] Whitefly nymphs infected with the fungi were stained with fluorescent indiacetate (FDA) and microscopically observed under blue light at a wavelength of 450-490 nm. [Results] mination of conidia on the cuticle of nymphs and penetration of the cuticle by fungal germ tubes were observed within 12 h and 24 h post-inoculation, respectively. Observation both with the naked eye and under fluorescent microscopy confirmed the concurrence of the production of red pigment and the multiplication of hyphal bodies in infected nymphs. [Conclusion] The new method allows the process of infection of B. tabaci by B.bassiana to be observed under fluorescent microscopy and infected nymphs to be readily identified.