赤眼蜂Trichogramma不同品系在寄生能力等生物学特性上存在差异，而这些差异可能被用于优良品系的筛选。通过优化影响赤眼蜂不同品系ISSR-PCR的主要参数，建立适于鉴别赤眼蜂不同品系的ISSR-PCR 反应体系和扩增程序。在20 μL反应体系中，各反应物的最适含量为10×PCR Buffer 2.0 μL,MgCl₂ 2. 0 μL（25 mmol/L），dNTPs 1.6μL（2.5 mmol/L each），正反向引物各1 μL（25 μmol/L），DNA模板1.0μL,Taq酶0.4 μL（2.5 U/μL），和ddH₂O。ISSR-PCR的扩增程序为：95℃预变性5 min，94℃变性50 s，52℃退火1 min，72℃延伸1 min 20 s，35个循环，72℃延伸10 min。结果表明，在上述优化条件下，采用ISSR-PCR可实现对7个赤眼蜂优良品系的分子鉴定。该研究对于赤眼蜂优良品系的筛选具有潜在的应用价值。

Different Trichogramma strains are differentiated from each other in biological characteristics, such as parasitic capability, which might be used for screening of superior Trichogramma strains. Here Inter-Simple Sequence Repeats (ISSR)-PCR was used to investigate the genetic variation of 7 candidate strains, and the molecular markers specific to different strains were subsequently identified. By optimizing the main parameters, the optimal ISSR-PCR reaction conditions for Trichogramma were firstly established. The results showed that the optimum concentrations of different reagents in a total of 20 μL volume were: 2.0 μL MgCl₂ (20 mmol/L), 1.6 μL dNTPs (.5 mmol/L each), 1 μL ISSR primer (10 μmmol/L), 1.5 μL genomic DNA and 0.4 μL Taq DNA polymerase (2.5 U/μL). The suitable PCR procedures were determined to be: pre-denaturing at 95℃ for 5 min, followed by 35 cycles of denaturing at 94℃ for 50 s, annealing at 52℃ for 1min and extension at 72℃ for 1 min 20 s, with a final extension at 72℃ for 10 min. Under the optimal conditions, the 7 Trichogramma strains tested could be discriminated by using ISSR-PCR. It is therefore suggested that this technique is helpful in identification and screening of Trichogramma strains.