黑蜂王台病毒中国BQCV-JL1株5'UTR的序列分析
Analysis of the sequence of the 5'UTR region of the Chineseblack queen cell virus-JL1
杨 倩1, 2** 张 健3 宋战昀1*** 郑 言1 王向辉1隋佳辰1 王振国1
点击：1772次 下载：24次
DOI：10.7679/j.issn.2095-1353.2015.033
作者单位：1. 吉林出入境检验检疫局检验检疫技术中心，长春 130062；2. 吉林农业大学，长春 130118；3. 长春生物制品研究所有限责任公司，长春 130062
中文关键词：BQCV，5'UTR，RT-PCR，序列分析，进化树分析
英文关键词： BQCV, 5'UTR, RT-PCR, sequence analysis, phylogenetic tree
中文摘要：
蜜蜂黑蜂王台病毒（Black queen cell virus, BQCV）是引起蜜蜂蜜蜂黑蜂王台病的病原体，主要侵染蜜蜂蜂王幼虫。BQCV基因组为单正链RNA，包括5'端的ORF 1和3'端的ORF 2两个开放阅读框。分别编码非结构蛋白和结构蛋白。本实验室首次成功分离鉴定得到中国首株BQCV毒株，命名为中国BQCV-JL1株。该株全长为8 358 nt。【目的】 本文主要研究该株核苷酸序列位置为1~1 124 nt的片段，该段序列的1~545 nt为该毒株的5'UTR 区域。【方法】 通过Blast及DNAStar等软件对核苷酸及氨基酸序列进行分析。【结果】 5'UTR 区域的同源性为94%~99%，为高度保守序列。【结论】 经Mega5软件作多重序列对比分析得知，该中国BQCV-JL1株的5'UTR区域与其他毒株差异性很大，经分析推断原因为碱基的缺失与碱基置换。
英文摘要： Black queen cell virus (BQCV) is a pathogen that mainly infects the queen bee larvae. The BQCV genome has a single positive-stranded RNA and two open reading frames (ORF); ORF 1 at the 5 ' end and ORF 2 at the 3' end, encoding non-structural, and structural, proteins respectively. Our laboratory has successfully isolated and identified the first BQCV strain in China, named Chinese BQCV-JL1 strain, which consists of 8 358 nucleotides. [Objectives] To characterize BQCV nucleotide sequences between 1 nt and 1 124 nt, and specifically the fragment containing the 5'UTR region (1-545 nt). [Methods] Nucleotide and amino acid sequences were analyzed using Blast and DNAStar software. [Results] The 5'UTR region is highly conserved with homology between 94% and 99%. [Conclusion] Mega5 software for multiple sequence alignment analysis shows that the Chinese BQCV-JL1 strain is very different from other BQCV strains. We infer that this is because of base deletion and base replacement.