棉铃虫饲养技术与流程
Rearing the cotton bollworm Helicoverpa armigera in the laboratory
秦启联
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DOI：10.7679/j.issn.2095-1353.2015.055
作者单位：农业虫害鼠害综合治理研究国家重点实验室，中国科学院动物研究所，北京 100101
中文关键词：棉铃虫，人工饲养，技术流程
英文关键词：Helicoverpa armigera, artificial rearing, workflow
中文摘要：
本文介绍了一套简便、实用的棉铃虫Helicoverpa armigera常规人工饲养技术及饲养流程。1~3龄幼虫用养虫罐群体饲养，4龄至化蛹用24孔养虫盒单头饲养。成虫饲喂10%蜂蜜水，在成虫产卵笼中把卵产在脱脂纱布上。饲养过程中应重点关注成虫所处空间的湿度、卵和蛹表面的消毒，以及饲养器具和材料的消毒和灭菌、饲养环境光周期等技术要点。本文还对人工饲养过程中的一些关键问题进行了讨论。
英文摘要： A simplified and practical method of rearing the cotton bollworm Helicoverpa armigera under laboratory conditions is introduced. Neonate to 3rd instar larvae were collectively reared in a specially-designed plastic jug and higher instars were individually maintained in a 24-grid plastic box in which they were allowed to pupate. Several technical issues that influenced the efficiency of the rearing were identified. These were; relative humidity (RH), which affects both copulation and oviposition, the surface disinfection of the eggs and pupae, sterilization or disinfection of all apparatus and materials used in rearing, and photoperiod. Problems encountered during the rearing process are discussed.