Bax通道在斑蝥素诱导草地贪夜蛾Sf9 细胞凋亡中的作用
Effects of the Bax channel on cantharidin induced apoptosis of Spodoptera frugiperda Sf9 cells
崔高峰** 张鹏飞 郝少东 丁 凯 王进忠 张志勇***
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DOI：10.7679/j.issn.2095-1353.2015.075
作者单位：北京农学院植物科学技术学院
中文关键词：斑蝥素，草地贪夜蛾，Bax通道，细胞形态，琥珀酸脱氢酶，线粒体膜电位
英文关键词：cantharidin, Spodoptera frugiperda, Bax channel, cell morphology, succinate dehydrogenase, mitochondrial membrane potential
中文摘要：【目的】为明确Bax通道在斑蝥素诱导鳞翅目昆虫细胞凋亡过程中的作用。【方法】 本文利用Bax通道抑制法测定了斑蝥素诱导鳞翅目昆虫草地贪夜蛾Spodoptera frugiperda(J.
E. Smith)细胞系Sf9细胞凋亡过程中线粒体膜电位、线粒体琥珀酸脱氢酶活性及细胞形态等方面的影响。【结果】 Bax通道被抑制后，斑蝥素诱导造成的Sf9细胞线粒体膜电位的降低时间延迟，细胞形变率下降，但琥珀酸脱氢酶活性的下降未受影响。【结论】 Bax通道参与了斑蝥素引起的Sf9细胞线粒体膜电位改变和细胞形态变化，而与抑制线粒体有关能量代谢的酶无直接关系。
英文摘要：[Objectives] To investigate the role of
the Bax channel on cantharidin-induced cell apoptosis in lepidopteran insects [Methods] Bax channel blocker was used to determine the
roles of mitochondrial membrane potential, succinate dehydrogenase activity in
mitochondria, and cell morphology, on apoptosis of Sf9 cells derived from fall
the armyworm, Spodoptera frugiperda (J.E.
Smith) exposed to cantharidin stress. [Results] The results show that Bax channel blocker can delay the decrease of
mitochondrial membrane potential and reduce changes in cell morphology,
but fail to stop the decline of succinate dehydrogenase activity, in Sf9
cells after treatment with cantharidin. [Conclusion] Bax
related channels play a role in cantharidin-induced changes in
mitochondrial membrane potential and destruction of cell morphology. However,
the Bax channel does not appear to play a role in the suppression of enzyme
activity in mitochondria after cantharidin treatment.